Modulation of the p38 MAPK Pathway by Anisomycin Promotes Ferroptosis of Hepatocellular Carcinoma through Phosphorylation of H3S10

<div>Ferroptosis of HCC cells was stimulated by anisomycin (AN) through the p38 MAPK signaling pathway. (a) Cell death was measured by flow cytometry. Ferrostatin-1 (Ferr-1) and SB203580 (SB) were used to inhibit ferroptosis, thus rescuing cells. (b–d) The assays showed that the content of intracellular iron, lipid peroxidation (LPO), and malondialdehyde (MDA) in HCC cells increased after AN treatment (2.5 <i>μ</i>M for Hep3B or 5 <i>μ</i>M for HCCLM3). <span class="nowrap"><svg height="8.8423pt" id="M28" style="vertical-align:-0.2064009pt" version="1.1" viewbox="-0.0498162 -8.6359 32.2343 8.8423" width="32.2343pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M822 650H589L583 622C660 617 677 607 674 561C672 534 664 481 647 390L600 137H596L273 650H126L120 622C176 620 194 615 207 594C221 571 225 557 214 504L161 257C141 166 129 112 121 85C108 42 83 30 29 28L23 0H260L266 28C193 33 173 42 176 89C178 122 186 172 202 255L256 527H259L583 -8H612L690 390C708 481 720 535 728 558C744 603 756 619 816 622L822 650Z"></path></g><g transform="matrix(.013,0,0,-0.013,14.542,0)"><path d="M535 323V373H52V323H535ZM535 138V188H52V138H535Z"></path></g><g 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Oxidative Medicine and Cellular Longevity

fig5

Figure 5

Figure 5: Modulation of the p38 MAPK Pathway by Anisomycin Promotes Ferroptosis of Hepatocellular Carcinoma through Phosphorylation of H3S10 