Modulation of the p38 MAPK Pathway by Anisomycin Promotes Ferroptosis of Hepatocellular Carcinoma through Phosphorylation of H3S10

Figure 6

NCOA4 was upregulated in anisomycin- (AN-) induced ferroptosis. (a) The heatmap based on qRT-PCR indicated changes in ferroptosis-related genes in HCCLM3 after treatment with AN for 12 h. In the inhibitor group, cells were pretreated with SB203580 (SB) for 1 h before being treated with anisomycin (2.5 μM for Hep3B or 5 μM for HCCLM3) for 12 h. (b) The qRT-PCR results showed the mRNA expression levels of five ferroptosis-related genes (SLC40A1, FTH1, FTL, NCOA4, and SLC3A2) in different groups of Hep3B and HCCLM3 cells. ; , , and . (c) The protein level of NCOA4 was detected in HCC cells after AN treatment by immunofluorescence. (d) The accumulation of lipid-ROS was detected in NOCA4 knockdown or negative control (NC) HCCLM3 after 12 h AN treatment (left). The accumulation of lipid-ROS was detected in NOCA4 cDNA or pEX-6 carrier transfected HCCLM3 after 12 h AN treatment (right).

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