Klotho Ameliorates Vascular Calcification via Promoting Autophagy

Figure 2

Obvious aortic VC appears in Klotho-deficient mice. (a) Klotho deficiency leads to apparent aortic calcification in Von Kossa staining experiments. (b) The Ca content of the aorta significantly increases in the KO group compared with that in the WT group (). (c) The expression of Runx2 significantly increases in the KO group compared with that in the WT group in IHC experiments (). (d) Klotho deficiency significantly increases Runx2 protein and decreases α-SMA protein expression in the KO group compared with those in the WT group (). .

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