Klotho Ameliorates Vascular Calcification via Promoting Autophagy

Figure 4

Autophagy increases in the aorta of Klotho-deficient mice. (a) Klotho deficiency induces a significant increase in LC3B-II/I ratio and decreases P62 protein expression in the KO and HZ groups compared with those in the WT group (). (b, c) Klotho deficiency induces a drastic increase in LC3B puncta/cell and decreases P62 fluorescence intensity in the KO and HZ groups compared with those in the WT group (). (d) The employment of RA significantly enhances the LC3B-II/I ratio but inhibits P62 protein expression in the group compared with those in the KO group (). (e) The employment of CQ significantly increases the LC3B-II/I ratio and P62 protein expression in the group compared with those in the KO group (). .

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