Klotho Ameliorates Vascular Calcification via Promoting Autophagy

Figure 5

Autophagy protects against aortic VC in Klotho-deficient mice. (a) The use of RA significantly inhibits Runx2 protein expression and promotes α-SMA protein expression in the group compared with those in the KO group (). (b) The use of CQ significantly promotes Ruxn2 protein expression and inhibits α-SMA protein expression in the group compared with those in the KO group (). (c) In IHC experiments, the expression of Runx2 markedly decreases in the group and increases in the group compared with those in the KO group (). (d) The Ca content significantly decreases after the stimulation of RA but increases after the stimulation of CQ compared with those in the KO group (). .

(a)

(b)

(c)

(d)