Protective Effect of Photobiomodulation against Hydrogen Peroxide-Induced Oxidative Damage by Promoting Autophagy through Inhibition of PI3K/AKT/mTOR Pathway in MC3T3-E1 Cells

Figure 1

PBM enhanced the cellular activity of MC3T3-E1 cells under oxidative stress. (a) CCK-8 assay for cell survival. Relative cell survival of MC3T3 cells cultured with different concentrations of H₂O₂ (). (b, c) The apoptosis was measured by TUNEL assays. Quantification of TUNEL-positive cells in every group (). . (d) Total ROS were determined using DCFH-DA. MC3T3-E1 cells were cultured with 200 μmol/L H₂O₂ and treated with PBM for different time. Compared with the H₂O₂ group, the number of viable cells was significantly increased (). (e, f) PBM treatment with 200 μm hydrogen peroxide induced MC3T3-E1 cells. ROS fluorescence intensity decreased significantly. Quantification of the amounts of positive cells expressing ROS in per group (). . The value < 0.05 significance level was considered significant. mean vs. control. ^#mean vs. 200 μmol/L.

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