Protective Effect of Photobiomodulation against Hydrogen Peroxide-Induced Oxidative Damage by Promoting Autophagy through Inhibition of PI3K/AKT/mTOR Pathway in MC3T3-E1 Cells

Figure 2

PBM inhibited H₂O₂-induced apoptosis of MC3T3 cells. (a–c) Western blotting was performed to test the Bax and Bcl2 levels in the control, H₂O₂, and treated with PBM groups (). (d, e) Cell apoptosis assays via flow cytometry. The proportions of prematurely apoptotic cells were increased by H₂O₂ treatment, but PBM can mitigate this influence. (f–h) Western blotting analysis of Bax and Bcl2 levels in the H₂O₂, H₂O₂+PBM, and NAC group (). A value of 0.05 or 0.01 should be considered.

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