Research Article
Protective Effect of Photobiomodulation against Hydrogen Peroxide-Induced Oxidative Damage by Promoting Autophagy through Inhibition of PI3K/AKT/mTOR Pathway in MC3T3-E1 Cells
Figure 4
PBM enhanced the autophagy level induced by H2O2 in MC3T3 cells. (a–c) Western blot detection of LC3B and P62 expression levels in the control, H2O2, and PBM-treated groups (). (d) TEM was used to identify autophagosomes in MC3T3 cell treated with H2O2 and PBM, scale bar: 2 μm and 500 nm. (e, f) LC3B-positive punctate structures were determined by immunofluorescence (). ; values < 0.05 or 0.01.
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