Protective Effect of Photobiomodulation against Hydrogen Peroxide-Induced Oxidative Damage by Promoting Autophagy through Inhibition of PI3K/AKT/mTOR Pathway in MC3T3-E1 Cells

Figure 5

Autophagy inhibitors can reverse the impact of PBM on H₂O₂-induced MC3T3 cells. (a–c) Western blotting assays of the LC3B and P62 expression levels of in the H₂O₂, 3-MA, and treated with PBM groups (). (d–f) The levels of Bax and Bcl2 expressions in the H₂O₂, 3-MA, and treated with PBM groups () were measured by western blotting. (g, h) Western blotting was performed to assay the levels of Runx2, Osx, OPN, and OCN in the H₂O₂, 3-MA, and treated with PBM groups (). (i) Alphabetic staining and alizarin red staining of MC3T3 cells processed with H₂O₂, 3-MA, and PBM. The values < 0.05, 0.01, and 0.001 and the scale bar represents 200 μm.

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