Protective Effect of Photobiomodulation against Hydrogen Peroxide-Induced Oxidative Damage by Promoting Autophagy through Inhibition of PI3K/AKT/mTOR Pathway in MC3T3-E1 Cells

Figure 6

Participation of PI3K/AKT/mTOR pathway in PBM to promote H₂O₂-induced autophagy in MC3T3 cells. (a, b) Levels of p-PI3K, p-AKT, and p-mTOR exposure in the control, H₂O₂, and PBM-treated groups were detected by western blotting (). (c, d) Western blotting analysis and quantification of the levels of the p-PI3K, p-AKT, p-mTOR in H₂O₂, H₂O₂+PBM, and 740 Y-P+PBM group (). (e, f) LC3B-positive punctate structures were determined by immunofluorescence. The number of positive LC3B cells in the H₂O₂+PBM and 740 Y-P+PBM groups was calculated (). The scale bar shows 20 μm; a statistically significant difference is defined as .

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