Protective Effect of Photobiomodulation against Hydrogen Peroxide-Induced Oxidative Damage by Promoting Autophagy through Inhibition of PI3K/AKT/mTOR Pathway in MC3T3-E1 Cells

<table class="table-group" id="tab2"><tr><td><table class="table"><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr class="thead"><td class="align_left">Genes</td><td class="align_center">Forward primer sequence (5<svg height="7.86364pt" id="M3" style="vertical-align:-0.04981995pt" version="1.1" viewbox="-0.0498162 -7.81382 4.53621 7.86364" width="4.53621pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M295 534L291 558C277 573 202 603 180 597L75 72L120 51L295 534Z"></path></g></svg>–3<span class="nowrap"><svg height="7.86364pt" id="M4" style="vertical-align:-0.04981995pt" version="1.1" viewbox="-0.0498162 -7.81382 4.53621 7.86364" width="4.53621pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M295 534L291 558C277 573 202 603 180 597L75 72L120 51L295 534Z"></path></g></svg>)</span></td><td class="align_center">Reverse primer sequence (5<span class="nowrap"><svg height="7.86364pt" id="M5" style="vertical-align:-0.04981995pt" version="1.1" viewbox="-0.0498162 -7.81382 4.53621 7.86364" width="4.53621pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M295 534L291 558C277 573 202 603 180 597L75 72L120 51L295 534Z"></path></g></svg>-</span>3<span class="nowrap"><svg height="7.86364pt" id="M6" style="vertical-align:-0.04981995pt" version="1.1" viewbox="-0.0498162 -7.81382 4.53621 7.86364" width="4.53621pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M295 534L291 558C277 573 202 603 180 597L75 72L120 51L295 534Z"></path></g></svg>)</span></td></tr><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr><td class="align_left">RUNX2</td><td class="align_center">GAACCAAGAAGGCACAGACAGA</td><td class="align_center">GGCGGGACACCTACTCTCATAC</td></tr><tr><td class="align_left">Osterix</td><td class="align_center">GATGGCGTCCTCTCTGCTT</td><td class="align_center">TATGGCTTCTTTGTGCCTCC</td></tr><tr><td class="align_left">Osteocalcin</td><td class="align_center">ACCATCTTTCTGCTCACTCTGCT</td><td class="align_center">CCTTATTGCCCTCCTGCTTG</td></tr><tr><td class="align_left">Osteopontin</td><td class="align_center">TACGACCATGAGATTGGCAGTGA</td><td class="align_center">TATAGGATCTGGGTGCAGGCTGTAA</td></tr><tr><td class="align_left"><i>β</i>-Actin</td><td class="align_center">CATCCGTAAAGACCTCTATGCCAAC</td><td class="align_center">ATGGAGCCACCGATCCACA</td></tr><tr class="table-tr"><td colspan="3"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

<div>Primer sequences used in RT-quantitative PCR.</div>

Oxidative Medicine and Cellular Longevity

tab2

Table 2

Table 2: Protective Effect of Photobiomodulation against Hydrogen Peroxide-Induced Oxidative Damage by Promoting Autophagy through Inhibition of PI3K/AKT/mTOR Pathway in MC3T3-E1 Cells 

Table 2 | Protective Effect of Photobiomodulation against Hydrogen Peroxide-Induced Oxidative Damage by Promoting Autophagy through Inhibition of PI3K/AKT/mTOR Pathway in MC3T3-E1 Cells 