Upregulated circTMEM59 Inhibits Cell Growth and Metastasis by miR-668-3p/ID4 Axis in Colorectal Cancer

Figure 1

CircTMEM59 validation and expression in CRC cells and tissues. (a) Volcano plot analysis of circTMEM59 expression in CRC from the GEO database (GSE142837). (b) circTMEM59 expression in 100 paired CRC tissues and adjacent normal tissues. (c) circTMEM59 expression in different tumor size of CRC. (d) CircTMEM59 expression in CRC cell lines and NCM460 cell lines. (e, f) Subcellular localization of circTMEM59 in CRC cells was analyzed by qRT-PCR and FISH (scale bar: 20 μm for FISH assay). (g) CircTMEM59 and linear TMEM59 expression levels were detected after RNase R treatment in CRC cells. (h) PCR and agarose gel electrophoresis confirmed the circular formation of circTMEM59, using divergent and convergent primers in gDNA and cDNA of HT29. GAPDH was used as a negative control. (i) Kaplan-Meier analysis of overall survival (OS) and disease-free survival (DFS) of CRC patients according to circTMEM59 expression levels. (j) The IHC of ki-67 and ID4 in the adjacent tissues, high expression circTMEM59 of tumors, and low expression circTMEM59 of tumors (scale bar: 100 μm). Data represent the . Student’s -test was used to determine statistical significance: , , , and .

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