Upregulated circTMEM59 Inhibits Cell Growth and Metastasis by miR-668-3p/ID4 Axis in Colorectal Cancer

Figure 2

Overexpressed circTMEM59 inhibits cell proliferation and metastasis of CRC in vitro. (a) qRT-PCR analysis of circTMEM59 in HT29 treated with sh-circTMEM59 and DLD1 cells treated with LV-circTMEM59. (b) Effects of downregulated and upregulated circTMEM59 on proliferation in CRC cell lines were detected by CCK-8 assay. (c) Colony formation assays were conducted to measure cell proliferation ability in HT29 and DLD1 cells. (d) Cell proliferation of CRC cells measured by EDU staining. μm. (e) Effects of circTMEM59 on regulating cell cycle were detected by flow cytometry. (f) The apoptotic rates were detected by flow cytometry in circTMEM59 knockdown or overexpression cells. (g) The migration and invasion abilities were detected by Transwell assays in circTMEM59 knockdown or overexpression cells (scale bar: 100 μm). (h) The IHC of ID4, Bcl-2, caspase-3, CDK4, cyclin D1, E-cadherin, and Vimentin in the low expression circTMEM59 of tumors and high expression circTMEM59 of tumors (scale bar: 100 μm). Data represent the . Two-way ANOVA and Student’s -test was used to determine statistical significance: , , and .

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