Upregulated circTMEM59 Inhibits Cell Growth and Metastasis by miR-668-3p/ID4 Axis in Colorectal Cancer

Figure 7

The regulation of circTMEM59 on CRC cells is mediated by ID4. (a) Expression of ID4 was confirmed by western blot in cotransfected CRC cell lines. (b–d) CCK-8 assays, EdU assays, and colony formation assays confirmed that ID4 mediated the effects of circTMEM59 on proliferation of CRC cells. (e) ID4 overexpression facilitated cell apoptosis of circTMEM59-downregulating of HT29 cells, while ID4 reduction inhibited cell apoptosis of circTMEM59-overexpressing of DLD1 cells. (f) Effects of circTMEM59 and ID4 on regulating cell cycle in cotransfected CRC cell lines. (g) The migration and invasion abilities were detected by Transwell assays in cotransfected CRC cell lines. (h) Western blot analysis confirmed that circTMEM59 inhibits cell cycle transition and cell metastasis and promotes cell apoptosis through regulating miR-668-3p of CRC. (i) Immunoblot analysis indicated that circTMEM59 restrains cell cycle transition and cell metastasis and facilitates cell apoptosis through regulating ID4 via sponging miR-668-3p of CRC. Data represent the . Two-way ANOVA and Student’s -test were used to determine statistical significance: , , and .

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