The Combination of Rhodosin and MMF Prolongs Cardiac Allograft Survival by Inhibiting DC Maturation by Promoting Mitochondrial Fusion

Figure 2

Effect of Rho and combination of Rho with low dose of MMF on the quantities of CD4⁺ and CD8⁺ T cells. The proportions of CD4⁺ and CD8⁺ T cells in the spleens and draining lymph nodes were determined by flow cytometry on day 5 after heart transplantation ( mice per group). (a) Macroscopic appearances of spleens and lymph nodes isolated from control group and MMF group, Rho group, and combination treatment group on day 5 after transplantation. (b) The weight (g) of spleens from different groups. (c) The proportions of CD4⁺ and CD8⁺ T cells in the spleens were determined by flow cytometry on day 5 after transplantation ( mice per group). (d) The proportions of CD4⁺ and CD8⁺ T cells in the draining lymph nodes were determined by flow cytometry on day 5 after transplantation ( mice per group). (e, f) Combination treatment mice showed low frequency of CD4⁺ T effector cells (CD4⁺CD44^hiCD62L^low) in spleens and draining lymph nodes. (g) The quantitative analysis of the CD4⁺ T, CD8⁺ T, CD4⁺ Teff cells in spleens and draining lymph nodes by GraphPad Prism 8.0. Data of histograms are presented as from six independent experiments (, , mice per group versus the control group).

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