Celastrol Protects against Cerebral Ischemia/Reperfusion Injury in Mice by Inhibiting Glycolysis through Targeting HIF-1α/PDK1 Axis

Figure 2

Celastrol decreased the level of glycolysis-related factors in ischemic cerebral cortex. QRT-PCR was used to detect the mRNA expression of LDHA (a), GLUT1 (b), and HK2 (c) (). Western blot was used to measure the protein expression of LDHA (d, e), GLUT1 (d, f), and HK2 (d, g) (). Lactate production (h), ATP level (i), and glucose content (j) were shown in the graphs, respectively (). The data are presented as . , , , and compared with the sham group; , , , and compared with the I/R group.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)