Fucoxanthin Attenuates Oxidative Damage by Activating the Sirt1/Nrf2/HO-1 Signaling Pathway to Protect the Kidney from Ischemia-Reperfusion Injury

Figure 4

Sirt1, Nrf2, and HO-1 protein expression levels were upregulated by fucoxanthin. The H/R model was established by hypoxia for 12 hours and reoxygenation for 6 hours. The HK-2 cells were treated with 5 μM fucoxanthin for 24 hours and then experienced H/R. (a)–(d) Sirt1, Nrf2, and HO-1 protein expression and their quantitative analysis. (e) and (f) Representative images of Sirt1 immunofluorescence staining (magnification ×400; ) and their quantitative analysis. (g) and (h) Representative images of Nrf2 immunofluorescence staining (magnification ×400; ) and its quantitative analysis of cytoplasmic and nuclear expression. Values are expressed as , . compared with control group, ^# compared with group.

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