Fucoxanthin Attenuates Oxidative Damage by Activating the Sirt1/Nrf2/HO-1 Signaling Pathway to Protect the Kidney from Ischemia-Reperfusion Injury

Figure 5

Inhibition of Sirt1 reversed the role of fucoxanthin in protecting HK-2 cells from H/R injury. The H/R model was established by hypoxia for 12 hours and reoxygenation for 6 hours. The HK-2 cells were treated with 5 μM fucoxanthin, 20 μM EX527, si-NC, or si-Sirt1 for 24 hours and then experienced H/R. (a)–(d) Sirt1, Nrf2, and HO-1 protein expression and their quantitative analysis. (e) and (f) SOD1 protein expression and its quantitative analysis. (g) and (h) Representative images of DCFH-DA staining (magnification ×100; ) and their quantitative analysis. (i)–(l) Expression of apoptosis-related proteins Bcl-2, Bax, cleaved caspase-3, and their quantitative analysis. (m) and (n) Percentage of apoptotic cells detected by flow cytometry. Values are expressed as , . compared with HR group, ^# compared with group, ^$ compared with group.

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