Fucoxanthin Attenuates Oxidative Damage by Activating the Sirt1/Nrf2/HO-1 Signaling Pathway to Protect the Kidney from Ischemia-Reperfusion Injury

Figure 7

EX527 eliminated the protective effect of fucoxanthin on renal I/R injury in mice. The I/R model was established with 45 minutes of ischemia and 24 hours of reperfusion. Mice were treated with 100 mg/kg of fucoxanthin or 5 mg/kg of EX527. (a) and (b) SOD1 protein expression. (c) and (d) Representative images of DHE staining (magnification × 400; ) and their quantitative analysis. (e)–(h) Expression of apoptosis-related proteins Bcl-2, Bax, cleaved caspase-3, and their quantitative analysis. (i) and (j) Representative images of TUNEL staining (magnification × 400; ) and their quantitative analysis. Apoptotic cells were detected by TUNEL (red). Values are expressed as , . compared with IR group, ^# compared with group.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)