miR-154-5p Affects the TGFβ1/Smad3 Pathway on the Fibrosis of Diabetic Kidney Disease via Binding E3 Ubiquitin Ligase Smurf1

Figure 3

Expression of miR-154-5p in primary cells and cell lines. Rat glomerular isolation (a). Morphology extraction of primary mesangial cells (PMCs) and primary proximal tubular cells (PPTCs, b) with scale bar 50 μm. miR-154-5p in high-glucose-cultured PMCs and PPTCs (c) as well as RMCs at different time points (d). CCK-8 cell proliferation in RMCs at different time points (e). Levels of VEGF, α-SMA, and FN in RMC supernatant (f). Protein expression in high-glucose-cultured RMCs (g). NG: normal glucose; HM: high mannitol; HG: high glucose. vs. NC, . samples/group.

(a)

(b)

(c)

(d)

(e)

(f)

(g)