Research Article

miR-154-5p Affects the TGFβ1/Smad3 Pathway on the Fibrosis of Diabetic Kidney Disease via Binding E3 Ubiquitin Ligase Smurf1

Figure 7

miR-154-5p influences Smurf1-mediated ubiquitination of Smad3. miRNA expression (a); CCK-8 cell proliferation (b); related protein Smurf1, pSmad3/Smad3, and ubiquitin concentration (c); luciferase verification for the binding of rno-miR-154-5p and rno-Smurf1 3 UTR (d) after pretreatment with miR-154-5p inhibitor and Smurf1 siRNA. vs. Group 1, ; #vs. Group 2, ; &vs. Group 3, . samples/group.