Selenium Attenuates TBHP-Induced Apoptosis of Nucleus Pulposus Cells by Suppressing Mitochondrial Fission through Activating Nuclear Factor Erythroid 2-Related Factor 2

Figure 1

Se alleviated TBHP-induced cytotoxicity and restored mitochondrial function in NPCs. (a, b) CCK-8 result of the NPCs viability with different concentrations of SS or Se-Met for 24 h. (c) CCK-8 result of the NPCs viability with different concentrations of TBHP for 6 h. (d, e) CCK-8 result of NPCs with TBHP (100 μM) and different concentrations of SS and Se-Met, respectively. (f) Fluorescence photomicrograph of live/dead cell staining of NPCs treated with Se and TBHP (scale bar: 100 μm). Green fluorescent indicates live cells and red fluorescent indicates dead cells. (g) Intracellular ROS measured by flow cytometry analysis. (h) Intracellular MDA levels in NPCs. (i, j) The expression of SOD2 in NPCs detected by western blot. (k) The mitochondrial ROS levels measured by flow cytometry analysis. (l) Mitochondrial membrane potential levels measured by flow cytometry analysis. (m) Quantitation of the ratio of JC-1 monomer. (n) Intracellular ATP content in NPCs. The data are represented as the from at least 3 independent experiments. and vs. control, and vs. the TBHP group. NS: no statistically significant difference.

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