Specific PFKFB3 Inhibitor Memorably Ameliorates Intervertebral Disc Degeneration via Inhibiting NF-κB and MAPK Signaling Pathway and Reprogramming of Energy Metabolism of Nucleus Pulposus Cells

Figure 3

KAN0438757 (KAN) inhibited the NF-κB signaling pathway and subsequent p-P65 nuclear translocation, as well as the MAPK signaling pathway. (a) The NF-κB signaling pathway protein expression levels after negative control, TNF-α, and TNF-α with 2.5 μM KAN treatment for 10 and 30 min in NPPs. (b) The MAPK signaling pathway protein expression levels after negative control, TNF-α, and TNF-α with 2.5 μM KAN treatment for 10 and 30 min in NPPs. (c) The gray levels of p-IKKα/β, p-IκBα, and p-P65 were quantified and normalized to their respective total protein using ImageJ. (d) The gray levels of p-ERK, p-JNK, and p-P38 were quantified and normalized to their respective total protein using ImageJ. (e) Nuclear translocation of p-P65 after negative control, TNF-α, and TNF-α with 2.5 μM KAN treatment for 15 min, visualized by immunofluorescence. Scale  μm and 5 μm. Data are expressed as , . ns: no significance; ; ; ; .

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