Specific PFKFB3 Inhibitor Memorably Ameliorates Intervertebral Disc Degeneration via Inhibiting NF-κB and MAPK Signaling Pathway and Reprogramming of Energy Metabolism of Nucleus Pulposus Cells

Figure 4

Metabolic reprogramming effects of KAN0438757 (KAN) on tumor necrosis factor-α- (TNF-α-) induced metabolism phenotype of nucleus pulposus primary (NPP) cells in vitro. (a) Normalized OCR of NPPs after negative control, TNF-α, and TNF-α with 2.5 μM KAN treatment for 24 hours. (b) Normalized ECAR of NPPs after negative control, TNF-α, and TNF-α with 2.5 μM KAN treatment for 24 hours. (c) Effects of KAN on basal respiration, maximal respiration, and spare respiratory capacity in OCR assay. (d) Effects of KAN on glycolysis, glycolytic capacity, and glycolytic reserve in ECAR assay. (e) Modified KAN reprogrammed the energy metabolism phenotype of TNF-α-inducted NPPs. Data are expressed as , . ns: no significance; ; ; ; .

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