Specific PFKFB3 Inhibitor Memorably Ameliorates Intervertebral Disc Degeneration via Inhibiting NF-κB and MAPK Signaling Pathway and Reprogramming of Energy Metabolism of Nucleus Pulposus Cells

Figure 5

KAN0438757 (KAN) ameliorated the progression of intervertebral disc degeneration (IVDD) in a rat tail disc puncture model in vivo. (a) The rat coccyx vertebrae X-ray images at 4 weeks after treatment from each group. (b) Quantitative analysis of disc height index (DHI, %) of IVDs in mice at 4 weeks after treatment in each group. . (c) Hematoxylin and eosin staining and safranin O-fast green staining of intervertebral discs (IVDs) from each group at 4 weeks after treatment. Scale  μm and 200 μm. (d) Quantitative analysis of histological scores of IVDs in mice at 4 weeks after treatment in each group. . (e) Interleukin- (IL-) 1β and Col2a1 immunofluorescence staining of IVDs from each group at 4 weeks after treatment. Scale  μm. (f, g) Quantitative analysis of IOD (IL-1β and Col2a1) of IVDs in mice at 4 weeks after treatment in each group. . Data are expressed as . ns: no significance; ; ; ; .

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