Knockdown of miR-214 Alleviates Renal Interstitial Fibrosis by Targeting the Regulation of the PTEN/PI3K/AKT Signalling Pathway

Figure 2

miR-214-3p directly targeted PTEN. (a) The 3-UTR of the human (hsa) and mouse (mmu) PTEN genes contained the miR-214-3p binding domain. (b) qPCR outcomes for the transfection efficiency of miR-214-3p mimics after 48 h transfection of the HK-2 cells. (c) The dual luciferase reporter analysis results after cotransfection of HK-2 cells using miR-214-3p/NC mimics plus WT/Mut PTEN 3-UTR. The data rendered are in terms of , , . (d) Western blotting detected PTEN levels after 48 h transfection of the TGF-β1 and control groups separately using mimic of miR-214-3p or NC. GAPDH served as an internal reference.

(a)

(b)

(c)

(d)