Dysregulation of EZH2/miR-138-5p Axis Contributes to Radiosensitivity in Hepatocellular Carcinoma Cell by Downregulating Hypoxia-Inducible Factor 1 Alpha (HIF-1α)

Figure 2

EZH2 is a direct target of miR-138-5p. (a) Venn diagram indicating the intersection of the number of target genes among the above four databases. (b) HEK293T cells were co-transfected with EZH2 3UTR-wt or EZH2 3UTR-mut with miR-138-5p mimics or NC. Following 48 h of transfection, luciferase activity was measured. (c) HepG2 and Hep3B were transfected with miR-138-5p mimic or NC for 24 h. The expression of miR-138-5p was determined by RT-qPCR analysis. The expression of U6 was used as an internal control. (d–e) HepG2 and Hep3B were transfected with miR-138-5p mimic or NC for 48 h. The expression of EZH2 was determined by Western blotting and RT-qPCR analysis. GAPDH was used as a loading control. ; (compared to NC group or vector group); ns, no significance.

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