Dysregulation of EZH2/miR-138-5p Axis Contributes to Radiosensitivity in Hepatocellular Carcinoma Cell by Downregulating Hypoxia-Inducible Factor 1 Alpha (HIF-1α)

Figure 3

miR-138-5p is epigenetically regulated by EZH2. (a) Binding sites of primers used for the analysis of chromatin immunoprecipitation (ChIP) are indicated relative to the transcription start site. (b) Expression of EZH2 in HepG2-shEZH2 and HepG2-NC groups. (c) The HepG2-NC and HepG2-shEZH2 were cultured and subjected to ChIP using H3K27me3 antibodies, or as control, normal human IgG. The precipitated chromatin was analyzed by RT-qPCR for the abundance of region PP1&PP2 upstream of the miR-138-5p gene. Values were normalized to chromatin levels in 1% input samples. (d) Total RNA of HepG2-shEZH2 was isolated from cells and the miR-138 level was quantified by RT-qPCR (E) Hep3B was treated with EZH2 overexpression plasmid and total RNA was isolated from cells. The miR-138-5p level was quantified by RT-qPCR. ; (compared to NC group or vector group).

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