Dysregulation of EZH2/miR-138-5p Axis Contributes to Radiosensitivity in Hepatocellular Carcinoma Cell by Downregulating Hypoxia-Inducible Factor 1 Alpha (HIF-1<i>α</i>)

<table class="table-group" id="tab1"><tr><td><table class="table"><tr><td class="thead-hr" colspan="2"><hr/></td></tr><tr class="thead"><td class="align_left">Gene</td><td class="align_center">Sequences (5<span class="nowrap"><svg height="7.86364pt" id="M1" style="vertical-align:-0.04981995pt" version="1.1" viewbox="-0.0498162 -7.81382 4.53621 7.86364" width="4.53621pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M295 534L291 558C277 573 202 603 180 597L75 72L120 51L295 534Z"></path></g></svg>-</span>3<span class="nowrap"><svg height="7.86364pt" id="M2" style="vertical-align:-0.04981995pt" version="1.1" viewbox="-0.0498162 -7.81382 4.53621 7.86364" width="4.53621pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M295 534L291 558C277 573 202 603 180 597L75 72L120 51L295 534Z"></path></g></svg>)</span></td></tr><tr><td class="thead-hr" colspan="2"><hr/></td></tr><tr><td class="align_left">miR-138-5p-F</td><td class="align_center">TGACCGAGCTGGTGTTGTG</td></tr><tr><td class="align_left">miR-138-5p-R</td><td class="align_center">CAGAGCAGGGTCCGAGGTA</td></tr><tr><td class="align_left">U6 snRNA-F</td><td class="align_center">CGCTTCGGCAGCACATATAC</td></tr><tr><td class="align_left">U6 snRNA-R</td><td class="align_center">TTCACGAATTTGCGTGTCATC</td></tr><tr><td class="align_left">EZH2-F</td><td class="align_center">TGGTCTCCCCTACAGCAGAA</td></tr><tr><td class="align_left">EZH2-R</td><td class="align_center">TCATCTCCCATATAAGGAATGTTATG</td></tr><tr><td class="align_left">GAPDH-F</td><td class="align_center">GTGAAGGTCGGAGTCAACG</td></tr><tr><td class="align_left">GAPDH-R</td><td class="align_center">TGAGGTCAATGAAGGGGTC</td></tr><tr><td class="align_left">HIF-1<i>α</i>-F</td><td class="align_center">TAAGTTCTGAACGTCGAAAAGA</td></tr><tr><td class="align_left">HIF-1<i>α</i>-R</td><td class="align_center">CAGCATCCAGAAGTTTCCTC</td></tr><tr><td class="align_center" colspan="2"><hr/></td></tr><tr><td class="align_left" colspan="2">ChIP-qPCR primer</td></tr><tr><td class="align_left">miR-138-5p-1-F</td><td class="align_center">CCATTGTTTCTCTGCACCCC</td></tr><tr><td class="align_left">miR-138-5p-1-R</td><td class="align_center">GTGGCTCCTCCGGTTTTGAG</td></tr><tr><td class="align_left">miR-138-5p-2-F</td><td class="align_center">CACCTGGCTGGGAGTTCTT</td></tr><tr><td class="align_left">miR-138-5p-2-R</td><td class="align_center">CTATTGCAGTCCTGGTCCTC</td></tr><tr class="table-tr"><td colspan="2"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

<div>Sequences of the primers used for RT-qPCR and ChIP-qPCR.</div>

Oxidative Medicine and Cellular Longevity

tab1

Table 1

Table 1: Dysregulation of EZH2/miR-138-5p Axis Contributes to Radiosensitivity in Hepatocellular Carcinoma Cell by Downregulating Hypoxia-Inducible Factor 1 Alpha (HIF-1<i>α</i>) 

Table 1 | Dysregulation of EZH2/miR-138-5p Axis Contributes to Radiosensitivity in Hepatocellular Carcinoma Cell by Downregulating Hypoxia-Inducible Factor 1 Alpha (HIF-1α) 