Naringin protects human nucleus pulposus cells against TNF-α-induced inflammation, oxidative stress, and loss of cellular homeostasis by enhancing autophagic flux via AMPK/SIRT1 activation

Figure 4

Naringin inhibits TNF-α-induced human NP cell apoptosis. (a) MitoTracker Red CMXRos fluorescence under fluorescence microscope (). (b) Annexin-V/PI staining to detect the rate of apoptosis in human NP cells. (c) The protein levels of C-caspase-3, Bax, and Beclin-2 in cells were measured by Western blotting. The data represent the . Significant differences between groups are indicated as , , and , .

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