Naringin protects human nucleus pulposus cells against TNF-α-induced inflammation, oxidative stress, and loss of cellular homeostasis by enhancing autophagic flux via AMPK/SIRT1 activation

Figure 5

Naringin ameliorates ECM degradation in TNF-α-induced human NP cells. (a) Alcian blue staining in human NP cells under inverted microscope (scale bar: 100 μm). (b) Collagen II staining in human NP cells under inverted microscope (scale bar: 100 μm). (c, d) The protein levels of SOX-9, aggrecan, collagen II, MMP-3, and ADAMTS-4 were measured by Western blotting. The data represent the . Significant differences between groups are indicated as , , and , .

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