Naringin protects human nucleus pulposus cells against TNF-<i>α</i>-induced inflammation, oxidative stress, and loss of cellular homeostasis by enhancing autophagic flux via AMPK/SIRT1 activation

<div>Regulation of autophagy in human NP cells cotreated with naringin. (a) Morphology of autophagosomes and autolysosomes was detected by transmission electron microscopy (×30000) in human NP cells (as indicated by black arrow). (b) The representative LC3 expression was detected by the immunofluorescence (scale bar: 50 <i>μ</i>m). (c) The protein levels of LC3, Beclin-1, and P62 in cells were measured by Western blotting. 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Oxidative Medicine and Cellular Longevity

fig6

Figure 6

Figure 6: Naringin protects human nucleus pulposus cells against TNF-<i>α</i>-induced inflammation, oxidative stress, and loss of cellular homeostasis by enhancing autophagic flux via AMPK/SIRT1 activation 

Figure 6 | Naringin protects human nucleus pulposus cells against TNF-α-induced inflammation, oxidative stress, and loss of cellular homeostasis by enhancing autophagic flux via AMPK/SIRT1 activation 