Autologous Bioactive Compound Concentrated Growth Factor Ameliorates Fistula Healing of Anal Fistula in a Pig Model and Promotes Proliferation and Migration of Human Skin Fibroblasts via Regulating the MEK/ERK Pathway

Figure 1

Porcine anal fistulae model creation process using rubber ligation surgery and treatment. (a) The marginal ear vessels injection with propofol at 2 mg/kg. (b) A 2 cm minimal incision was made in the anus at 3, 9, and 12 o’clock positions, and ligated rubber was used. (c) Directly cross through the anal rectal mucosa with the hemostatic clamp. (d) Rubber ligation was fixed to the fistula. (e) 10 ml blood sample was corrected from the pig. (f) Concentrated growth factor (CGF) was prepared from the pig itself. (g) CGF was used to treat the fistula at the 9 o’clock position. (h) CGF treatment for 2 weeks. (i) CGF treatment for 4 weeks. (j) B-mode ultrasound diagnosis imaging evaluation of created fistulas. (k) Posttreatment specimens of anal fistulae were stained with H&E and visualized at 20× and 40×.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)

(k)