Autologous Bioactive Compound Concentrated Growth Factor Ameliorates Fistula Healing of Anal Fistula in a Pig Model and Promotes Proliferation and Migration of Human Skin Fibroblasts via Regulating the MEK/ERK Pathway

Figure 10

Knock-down of ERK effectively reverses CGF-induced activation of cell proliferation and migration ability in HSF cells. (a) Expression of ERK1/2 was confirmed by Western blot in HSF cells transfected with siRNA-NC or siRNA-ERK. (b, c) Cell proliferation was determined in 20% CGF-treated HSF cells following transfection with siRNA-ERK. (d) Cell migration assays were performed in 20% CGF-treated HSF cells following transfection with siRNA-ERK using wound healing assays. (e, f) The protein levels of Col1A1, Col3A1, TIMP-1, MMP-3, C-fos, E-cadherin, vimentin, p-MEK1/2, MEK1/2, p-ERK1/2, and ERK1/2 were detected in 20% CGF-treated HSF cells following transfection with siRNA-ERK by using Western blot assays. , vs. blank group; ^#, ^## vs. 20% CGF+siRNA-NC group.

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