Attenuation Effect of Salvianolic Acid B on Testicular Ischemia-Reperfusion Injury in Rats

Figure 4

Light microscopy of hematoxylin and eosin stained sections of rat testicular tissues in control, ischemia-reperfusion, and salvianolic acid B-treated groups (original magnification, ×200). (a) Bilateral testes in the control group and contralateral nontorsional testes in ischemia-reperfusion and salvianolic acid B-treated groups all exhibited normal diameter of seminiferous tubule, germ cell layer number, and spermatogenesis from spermatogonia in tubular basal membrane to mature spermatozoa (↑) in tubular lumen. The germinal epithelium of seminiferous tubule left an open lumen in tubular center. (b) The diameter of seminiferous tubule (↑) and germ cell layer number (▲) were lower in ipsilateral torsional testes of ischemia-reperfusion group versus control group (). Mature spermatozoa were completely absent in seminiferous tubule. (c) Ipsilateral testes in the salvianolic acid B-treated group showed that histologic structure of seminiferous tubule was close to normal. However, in tubular lumen, there were many sloughed germinal cells (↑), which easily blocked up tubular lumen.

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