Quercetin Protects against MPP⁺/MPTP-Induced Dopaminergic Neuron Death in Parkinson’s Disease by Inhibiting Ferroptosis

Figure 1

QCT significantly inhibited Erastin- and RSL3-induced ferroptosis in M17, PC12, and SH-SY5Y cells. (a–d) Viabilities of M17, PC12, and SH-SY5Y cells after treatment with QCT (10 μM), Fer-1 (1 μM), and Lip-1 (200 nM) for 1 h, respectively, followed by Erastin (1 μM, 24 h) or RSL3 (1 μM, 12 h) treatments (). (e) Representative transmission electron microscope images for M17 cells treated with or without QCT (10 μM) or Fer-1 (1 μM, 1 h), followed by Erastin (2 μM, 12 h) treatment. Red arrows indicate shrunken mitochondria. Scale bars as indicated. (f, g) The M17 cells were treated with QCT (10 μM) or Fer-1 (1 μM) for 1 h, followed by Erastin (2 μM) for 24 h, and the lipid ROS assayed by flow cytometry using C11-BODIPY (). (h) Representative immunoblots of the GPX4 protein from M17 cells treated with or without QCT (10 μM, 1 h), or Fer-1 (1 μM, h), followed by Erastin (1 μM, 12 h) treatment. (i) Quantification of GPX4 protein levels (). Data are presented as . One-way ANOVA followed by Tukey’s post hoc test. , , and .

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