Quercetin Protects against MPP⁺/MPTP-Induced Dopaminergic Neuron Death in Parkinson’s Disease by Inhibiting Ferroptosis

Figure 2

QCT inhibited Erastin-induced ferroptosis in M17 cells by activating the Nrf2 pathway. (a) Representative immunoblots for SLC7A11, FTH, and GPX4 proteins from M17 cells treated with or without QCT (10 μM, 1 h), followed by Erastin (1 μM, 12 h) treatment. (b–d) Quantification of SLC7A11, FTH, and GPX4 protein levels (). (e, f) Representative immunoblots for nuclear and total Nrf2 protein levels from M17 cells treated with or without QCT (10 μM, 1 h), followed by Erastin (1 μM, 12 h) treatment. (f, g) Quantification of nuclear and total Nrf2 protein levels (). Immunofluorescence analysis of M17 cells treated with or without QCT (10 μM, 1 h), followed by Erastin (1 μM, 12 h) treatment, stained with Nrf2 antibody (green) and DAPI (blue).  μm. Data are presented as . One-way ANOVA followed by Tukey’s post hoc test. , , and ; ns: no significant difference.

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