Quercetin Protects against MPP⁺/MPTP-Induced Dopaminergic Neuron Death in Parkinson’s Disease by Inhibiting Ferroptosis

Figure 3

QCT inhibited MPP⁺-induced ferroptosis in PC12 cells by activating the Nrf2 pathway. (a) Viabilities of PC12 cells treated with 0.25, 0.5, 0.75, and 1 mM MPP⁺ for 24 h or 48 h (). (b) Viability of PC12 cells treated with or without Fer-1 (1 μM, 1 h), followed by MPP⁺ (0.5 mM, 24 h) treatment (). (c) Viability of PC12 cells treated with or without QCT (10 μM, 1 h), followed by MPP⁺ (0.5 mM, 24 h) treatment (). (d) Representative immunoblots for SLC7A11 and GPX4 proteins from PC12 cells treated with or without QCT (10 μM, 1 h), followed by MPP⁺ treatment (0.5 mM, 24 h). (e, f) Quantification of SLC7A11 and GPX4 protein levels (). (g) Representative immunoblots for nuclear and total Nrf2 proteins from PC12 cells treated with or without QCT (10 μM, 1 h), followed by MPP⁺ (0.5 mM, 24 h) treatment. (h, i) Quantification of nuclear and total Nrf2 protein levels (). (j) Immunofluorescent analysis from PC12 cells treated with or without QCT (10 μM, 1 h), followed by MPP⁺ (0.5 mM, 24 h) treatment.  μm. Data are presented as . One-way ANOVA followed by Tukey’s post hoc test. , , and ; ns: no significant difference.

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