Quercetin Protects against MPP⁺/MPTP-Induced Dopaminergic Neuron Death in Parkinson’s Disease by Inhibiting Ferroptosis

Figure 5

Nrf2 knockdown compromised the protective effects of QCT. (a) Representative immunoblots for PC12 cells transfected with Ctrl siRNA or Nrf2 siRNA. (b) Quantification of Nrf2 protein levels (). (c) Cell viability was assayed for transfected PC12 cells subjected to QCT (10 μM, 1 h), before being treated with MPP⁺ (0.5 mM, 24 h) (). (d) Representative immunoblots for SLC7A11 and GPX4 proteins from PC12 cells transfected with Nrf2 siRNA for 48 h and then treated with QCT (10 μM, 1 h) before being subjected to MPP⁺ (0.5 mM, 24 h). (e, f) Quantification of SLC7A11 and GPX4 protein levels (). Data are presented as the . Student’s -test and one-way ANOVA followed by Tukey’s post hoc test. , , and .

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