Novel piRNA Regulates PIWIL1 to Modulate the Behavior of Placental Trophoblast Cells and Participates in Preeclampsia

Figure 6

(a) Images of immunohistochemical staining showing the localization of PIWIL1 in placental tissues from normal pregnancies and pregnancies with preeclampsia. (b) Quantitative analysis of PIWIL1 expression in the two groups (35 preeclampsia and 29 normal placentas). (c) PIWIL1 protein expression in placental tissues from normal pregnancies and pregnancies with preeclampsia as visualized by western blotting and quantitative analysis. (d) Expression of PIWIL1 in the human extravillous trophoblast cell line HTR-8/SVneo and choriocarcinoma cell lines JAR and JEG-3. (e) Overexpression and silencing of PIWIL1 in HTR-8/SVneo cells. The figure shows the following: representative PIWIL1 protein bands and quantitative analysis of protein expression in HTR-8/SVneo cells transfected with PIWIL1 siRNAs and a negative control siRNA (NC). PIWIL1 protein bands and quantitative analysis of protein expression in HTR-8/SVneo cells transfected with a PIWIL1-overexpression vector. , , and .

(a) Immunohistochemical staining

(b) Quantitative analysis

(c) PIWIL1 protein expression

(d) Expression of PIWIL1 in the human extravillous trophoblast cell line HTR-8/SVneo, JAR, and JEG-3

(e) Overexpression and silencing of PIWIL1 in HTR-8/SVneo cells