Hypoxia Enhances Glioma Resistance to Sulfasalazine-Induced Ferroptosis by Upregulating SLC7A11 via PI3K/AKT/HIF-1α Axis

Figure 5

PX-478 induced lipid peroxidation in the U87 and U251 cells. (a, b) Lipid peroxidation assessment in U87 and U251 cells after exposure to DMSO, PX-478, or PX-478+Lv-SLC7A11 measured by fluorescence microscope (a) and flow cytometry (b). (c) Quantitative analysis of the expression levels of MDA in the U87 and U251 cells after exposure to DMSO, PX-478, or PX-478+Lv-SLC7A11. MDA increased after treating with PX-478 and reversed in the presence of Lv-SLC7A11. (d) Transmission electron microscopy images of the U87 and U251 cells after exposure to DMSO, PX-478, or PX-478+Lv-SLC7A11. PX-478-treated cancer cells exhibited shrunken mitochondria with enhanced membrane density. Black arrow: normal mitochondria; white arrow: shrunken and high membrane density mitochondria. Quantitative analysis of mitochondrial length was listed on the right. (e, f) Cell apoptosis was detected by flow cytometry (e) and TUNEL fluorescence staining (f) in the U87 and U251 cells after exposure to DMSO, PX-478, SAS, PX-478+SAS, and PX-478+Lv-SLC7A11+SAS. The bar graph showed of 3 independent experiments. and .

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