Ginkgolic acid improves bleomycin-induced pulmonary fibrosis by inhibiting SMAD4 SUMOylation

Figure 3

Ginkgolic acid (GA) prevented pulmonary fibrosis by increasing SENP1 expression and activity in vivo. Wild-type (WT) mice and SENP1-knock out (KO) transgenic mice were challenged with bleomycin (BLM) through the airway and treated by intraperitoneal administration of GA after 24 h. Meanwhile, the control group was administered and treated with normal saline (n =8). (a) SUMO1, UBC9, and SENP1 protein levels in lung tissues from the different groups were detected by WB. (b) Images of representative HE and Masson staining of lung tissues from the indicated groups. Quantitative lung injury scores and Ashcroft scores are shown as box charts. Scale bar: 100 μm. (c) ELISA kits were used to detect levels of TGF-β1, TNF-α, and IL-6 in lung tissues of each group. Data are presented as the . , .

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