Sesamol Attenuates Neuroinflammation by Regulating the AMPK/SIRT1/NF-κB Signaling Pathway after Spinal Cord Injury in Mice

Figure 4

Sesamol regulates AMPK/SIRT1/NF-κB pathways in mice following SCI. (a) Western blot images showing protein expressions of p-AMPK, AMPK, SIRT1, p-p65, and p65 in the spinal cord of mice from uninjured, SCI, and sesamol-treated group at 3 dpi. (b–d) Quantitative analysis of western blot results from (a) ( per group). (e) Representative images showing that sesamol increased the fluorescence degree of SIRT1 (green) in activated microglia (established by IBA1, purple), and DAPI (blue) is for the nuclear staining (). (f) Sesamol reduced the fluorescence degree of p65 (red) in activated microglia (). (g, h) Quantification of the fluorescence degree of SIRT1 and p65 in activated microglia from (e) and (f), respectively. Data are represented as the ( per group). ^##, ^###, and ^####.

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