Clinical Relevance and Tumor Growth Suppression of Mitochondrial ROS Regulators along NADH:Ubiquinone Oxidoreductase Subunit B3 in Thyroid Cancer

Figure 4

The role of NDUFB3 overexpression in mitochondrial respiration and mitoROS generation. (a) Volcano plots were constructed using fold-change values and adjusted (NDUFB3^low versus NDUFB3^high). The red point in the plot represents the overexpressed mRNAs, and the blue point indicates the downregulated mRNAs with statistical significance. The plot of NDUFB3 is shown on the top, as indicated. (b) Volcano plots were constructed using enrichment score and FDR. The number of genes enriched in each KEGG pathway is shown in the top-left corner. In the enrichment result, or is considered to be enriched to a meaningful pathway. (c) GSEA histogram for the gene set “OXPHOS.” Real-time OCR tracing of BCPAP cells or C643 cells stably overexpressing NDUFB3 or empty vector control (). (d) Total cellular ATP concentration in BCPAP cells or C643 cells stably overexpressing NDUFB3 or empty vector control (). Statistical differences were determined by a two-tailed unpaired Mann–Whitney -test. ; . (e) Complex I activity was determined in mitochondria isolated from BCPAP cells or C643 cells overexpressing NDFUB3 or empty vector control (). Statistical differences were determined using a two-tailed unpaired Mann–Whitney -test. ; . (f) Representative flow cytometry analysis of NDUFB3 overexpression or its empty vector control showing respective mitoROS levels in BCPAP or C643 cells.

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