Attenuation by Time-Restricted Feeding of High-Fat and High-Fructose Diet-Induced NASH in Mice Is Related to Per2 and Ferroptosis

Figure 3

TRF suppresses the ferroptosis in NASH mice. (a) Acsl4 and Gpx4 protein levels were measured by western blotting, with GAPDH used as a control. (b) Hepatic mRNA levels for Acsl4, Gpx4, Aifm2, and Ptgs2 were measured by RT-qPCR; and GAPDH was used as a control. (c, d) Liver GSH and MDA concentrations were quantified with commercial kits. (e) Fluorescence staining showing lipid peroxidation (LPO) levels in the FA, FT, NA, and NT groups. The nuclei were labeled with DAPI (left), and LPO (middle) was labeled with C11-Bodipy (original magnification, 400x; scale bar, 50 μm). (f) Electron microscopic analysis of the morphologic changes in mitochondria; the white arrow indicates lipid droplets, and the black arrow indicates mitochondria (original magnification, 5000x; scale bar, 1 μm) (NA and NT, ; FA and FT, ). , , and ; ns: not significant.

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