Attenuation by Time-Restricted Feeding of High-Fat and High-Fructose Diet-Induced NASH in Mice Is Related to Per2 and Ferroptosis

<div>TRF reduces the iron accumulation in the serum and liver as induced by HFHFD. (a) Serum and liver iron concentrations were determined using a commercial kit to compare the iron overload levels among the four groups. (b) Hepatic mRNA levels for Irp1, Irp2, Tfr1, Tfr2, and Slc40a1 were measured by RT-qPCR, and GAPDH was used as a control. (c) Tfr1 protein levels were measured by western blotting, with GAPDH used as a control. 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Oxidative Medicine and Cellular Longevity

fig4

Figure 4

Figure 4: Attenuation by Time-Restricted Feeding of High-Fat and High-Fructose Diet-Induced NASH in Mice Is Related to Per2 and Ferroptosis 