Attenuation by Time-Restricted Feeding of High-Fat and High-Fructose Diet-Induced NASH in Mice Is Related to Per2 and Ferroptosis

<div>TRF and Per2 knockout promote the expression of PPAR<i>α</i>. (a) Hepatic mRNA levels for PPAR<i>α</i> in Per2<sup>△hep</sup> and Per2<sup>fl/fl</sup> mice were measured by RT-qPCR, and GAPDH was used as a control. (b) PPAR<i>α</i> protein levels in Per2<sup>△hep</sup> and Per2<sup>fl/fl</sup> mice were measured using western blotting, with GAPDH used as a control. (c) Hepatic mRNA levels for PPAR<i>α</i> in the NA, NT, FA, and FA groups were measured by RT-qPCR, and GAPDH was used as a control. (d) PPAR<i>α</i> protein levels in the NA, NT, FA, and FA groups were analyzed by western blotting, with GAPDH used as a control. Both Per2<sup>△hep</sup> and Per2<sup>fl/fl</sup> mice were fed with HFHFD to induce NASH. 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Oxidative Medicine and Cellular Longevity

fig9

Figure 9

Figure 9: Attenuation by Time-Restricted Feeding of High-Fat and High-Fructose Diet-Induced NASH in Mice Is Related to Per2 and Ferroptosis 