Research Article

AdipoR1 Regulates Ionizing Radiation-Induced Ferroptosis in HCC cells through Nrf2/xCT Pathway

Figure 4

AdipoR1 is involved in erastin-induced ferroptosis in HCC cells. (a–d) Lipid peroxidation and cell viability of erastin-treated MHCC-97H and HepG2 cells were assessed by flow cytometry using C11-BODIPY (a and b, vs Ctrl) and CCK-8 kit, respectively. (e–i) After treatment with erastin (20 μM) for 48 h, lipid peroxidation and cell viability were assessed in AdipoR1 knockdown MHCC-97H and HepG2 cells, respectively. (j and k) After treatment with erastin (20 μM) for various times (4 h, 8 h, 12 h, and 24 h), Western blot showed the protein expression of AdipoR1, xCT, and GPX4 in HCC cells. Data is presented as . , , , and .