Interaction between TRPML1 and p62 in Regulating Autophagosome-Lysosome Fusion and Impeding Neuroaxonal Dystrophy in Alzheimer’s Disease

Figure 3

Overexpression of TRPML1 promotes autophagosome-lysosome fusion and stimulates BDNF nuclear transport to block NAD in AD. (a) Protein expression of BDNF and the ratio of p-TrkB/TrkB and p-CREB/CREB in neurons, evaluated by Western blot analysis. (b) Changes of autolysosome formation in neurons identified by immunofluorescence staining. (c) Changes of BDNF nuclear translocation detected by immunofluorescence staining. (d) Length of axons in HT22 cells observed under an inverted microscope. Measurement data were described as the One-way ANOVA was used for comparisons among multiple groups, followed by Tukey’s post hoc test. The cell experiment was repeated 3 times independently. vs. untreated HT22 cells; ^# vs. cells treated with Aβ1-42; ^& vs. cells treated with oe-NC+Aβ1-42. ^{^} vs. cells treated with oe-TRPML1+Aβ1-42.

(a)

(b)

(c)

(d)