STING Induces Liver Ischemia-Reperfusion Injury by Promoting Calcium-Dependent Caspase 1-GSDMD Processing in Macrophages

Figure 3

Knockdown of STING in macrophages alleviated liver IRI. Mice were pretreated (i.v.) with AAV-STING-RNAi-F4/80-EGFP () 14 days before liver IRI modeling. A volume of nonspecific AAV (AAV-Ctrl-F4/80-EGFP) equal to the treatment volume was administered in the same manner.(a) KCs were isolated from the liver in each group, and then the expression of STING in KCs in each group was measured by Western blotting. (b) Relative STING expression of KCs in each group. (c) STING expression in each group was measured by immunohistochemistry (scale bar, 200 μm). (d, e) Serum levels of ALT and AST were measured. (f, g) H&E-stained sections of livers; average Suzuki scores were based on H&E-stained liver sections from different groups of mice (scale bar, 100 μm). All data are shown as the ( mice per group). , , and .

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